January 2025 · Stem Cell Reports
MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells
Pierre Osteil, Sarah Withey, Nicole Santucci, Nima Aryamanesh, Ian Pang, Nadiar Salehin, Zhaoxiang Cai, Ernst Wolvetang, Patrick P L Tam
Abstract
MIXL1 plays a critical role in endoderm differentiation. Here, we demonstrate that MIXL1 activation is a key determinant of the efficiency of definitive endoderm generation from human induced pluripotent stem cells (hiPSCs). By modulating MIXL1 expression, we show that lineage propensity can be re-wired, enhancing the differentiation potential toward endoderm lineages. This work provides insights for optimizing stem cell differentiation protocols for regenerative medicine applications.
BibTeX
@article{osteil2025osteil2025mixl1,
title = {{MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells}},
author = {Pierre Osteil and Sarah Withey and Nicole Santucci and Nima Aryamanesh and Ian Pang and Nadiar Salehin and Zhaoxiang Cai and Ernst Wolvetang and Patrick P L Tam},
journal = {Stem Cell Reports},
year = {2025}
}